Page 19 - InnoRenew CoE International Conference 2020, Integrating Sustainability and Health in Buildings
P. 19
Influence of Four Commercial Wood-surface Treatments on Mould-fungi
Growth in a Pure Culture
Olena S. Myronycheva1, Injeong Kim1, Peter Jacobsson2, Olov Karlsson1,
Margot Sehlstedt-Persson1, Dick Sandberg1
1 Luleå University of Technology, Wood Science and Engineering, olena.myronycheva@ltu.se, injeong.kim@ltu.se,
olov.karlsson@ltu.se, margot.sehlstedt-persson@ltu.se, dick.sandberg@ltu.se
2 Martinsons Byggsystem AB, peter.jacobsson@martinsons.se
The increased concern regarding mould on wood-based building materials has raised demand
for sustainable biocidal treatments to protect early contamination during the construction stage
of timber buildings. Providing surface protection for all types of wood-based construction
elements already at the construction site will reduce the mould-associated risk for not only
the construction elements but also for the indoor climate and dwellers at the use-stage of the
building. The purpose was to test the protective effect of commercial water-based treatments
containing different biocides on single mould fungi growth in pure culture.
Small specimens of Scots pine, sapwood and heartwood, and Norway spruce were treated with
four treatments, and a fungal test was performed in 90 mm Petri plates. Two samples (treated
and untreated control) placed on the plate with a distance between each other, and between, a
fungal inoculum placed. Five pure cultures of fungi species used in the study: Aureobasidium sp.,
Trichoderma sp., Aspergillus sp., Cladosporium sp., Penicillium sp. The specimens were exposed at
the temperature of 24°C and relative humidity of 90%.
The treatments contained biocides, i.e.: treatment 1: tetramethylol acetylenediurea and
io-dopropynyl butyl carbamate (IPBC); treatment 2: mixture of several biocides (IPBC,
benzisothiazolinone [BIT], methylisothiazolinone [MIT] and 5-chloro-2-methyl-1,2-thiazol-
3-one [CMIT/MIT mixture]); treatment 3: IPBC; and treatment 4: mixture of propiconazole and
IPBC.
The fungal growth was observed in the untreated samples after four days of incubation; the
specimens with treatment 4 attacked after eight days while treatment 1 and 3 had free from
fungal mycelia inhibition zone after 22 days of incubation. The moisture content after the test
was similar for treated samples in plates with Aureobasidium sp., Aspergillus sp., Cladosporium
sp., Penicillium sp. but was significantly higher for Trichoderma sp. The type of wood did not
influence fungal growth in comparison to the type of treatment.
Keywords: biocide, wood, surface treatment, fungi, mould
Acknowledgements: First Author gratefully acknowledges receiving funding from programme
Bioinnovation (IPOS-DP2) and from the Swedish Research Council for Environment, Agricultural
Sciences and Spatial Planning (FORMAS), the project “Fungal growth on modified wood-based
products under subarctic conditions” (2017-00419).
INTEGRATING SUSTAINABILITY AND HEALTH IN BUILDINGS THROUGH RENEWABLE MATERIALS
19
Growth in a Pure Culture
Olena S. Myronycheva1, Injeong Kim1, Peter Jacobsson2, Olov Karlsson1,
Margot Sehlstedt-Persson1, Dick Sandberg1
1 Luleå University of Technology, Wood Science and Engineering, olena.myronycheva@ltu.se, injeong.kim@ltu.se,
olov.karlsson@ltu.se, margot.sehlstedt-persson@ltu.se, dick.sandberg@ltu.se
2 Martinsons Byggsystem AB, peter.jacobsson@martinsons.se
The increased concern regarding mould on wood-based building materials has raised demand
for sustainable biocidal treatments to protect early contamination during the construction stage
of timber buildings. Providing surface protection for all types of wood-based construction
elements already at the construction site will reduce the mould-associated risk for not only
the construction elements but also for the indoor climate and dwellers at the use-stage of the
building. The purpose was to test the protective effect of commercial water-based treatments
containing different biocides on single mould fungi growth in pure culture.
Small specimens of Scots pine, sapwood and heartwood, and Norway spruce were treated with
four treatments, and a fungal test was performed in 90 mm Petri plates. Two samples (treated
and untreated control) placed on the plate with a distance between each other, and between, a
fungal inoculum placed. Five pure cultures of fungi species used in the study: Aureobasidium sp.,
Trichoderma sp., Aspergillus sp., Cladosporium sp., Penicillium sp. The specimens were exposed at
the temperature of 24°C and relative humidity of 90%.
The treatments contained biocides, i.e.: treatment 1: tetramethylol acetylenediurea and
io-dopropynyl butyl carbamate (IPBC); treatment 2: mixture of several biocides (IPBC,
benzisothiazolinone [BIT], methylisothiazolinone [MIT] and 5-chloro-2-methyl-1,2-thiazol-
3-one [CMIT/MIT mixture]); treatment 3: IPBC; and treatment 4: mixture of propiconazole and
IPBC.
The fungal growth was observed in the untreated samples after four days of incubation; the
specimens with treatment 4 attacked after eight days while treatment 1 and 3 had free from
fungal mycelia inhibition zone after 22 days of incubation. The moisture content after the test
was similar for treated samples in plates with Aureobasidium sp., Aspergillus sp., Cladosporium
sp., Penicillium sp. but was significantly higher for Trichoderma sp. The type of wood did not
influence fungal growth in comparison to the type of treatment.
Keywords: biocide, wood, surface treatment, fungi, mould
Acknowledgements: First Author gratefully acknowledges receiving funding from programme
Bioinnovation (IPOS-DP2) and from the Swedish Research Council for Environment, Agricultural
Sciences and Spatial Planning (FORMAS), the project “Fungal growth on modified wood-based
products under subarctic conditions” (2017-00419).
INTEGRATING SUSTAINABILITY AND HEALTH IN BUILDINGS THROUGH RENEWABLE MATERIALS
19
